Figure 4.

Immunoselected proteins of symplekin-containing particles present in the HSS fraction from Xenopus oocyte nuclei (A) and from egg extracts (B). The precipitates were solubilized in sample buffer, separated by SDS-PAGE and stained with Coomassie Brilliant Blue. The prominent protein bands (lane 2) were analyzed by peptide mass fingerprinting. In addition to symplekin, 3 out of 4 proteins of the CPSF complex were identified as CPSF-160, CPSF-100, and CPSF-73. Lane 1, reference proteins (molecular mass values are indicated on the left).