Figure 6.

Laser scanning confocal microscopy showing the result of a of double-labeling experiment, comparing here the localization of symplekin (A) with that of protein CPSF-100 (B) in a cryosection through a Xenopus laevis ovary. The corresponding merge picture is shown in (C), the corresponding phase contrast in (D). Note that the Cajal bodies in the nuclei are strongly positive for both symplekin (A) and CPSF-100 (B), as indicated by the yellow color in (C), whereas under the immunolocalization conditions applied here only a weak and diffuse reaction is seen in the karyo- and the cytoplasm. Bar, 100 μm.