Figure 8.

Characterization of symplekin-containing particles in cytoplasmic extracts of enucleated Xenopus oocytes by sucrose gradient centrifugation. Extracts from 40 ooplasms have been subjected to sucrose gradient centrifugation, as described in Figure 5, using catalase (11.3S, fraction 6), thyroglobulin (16.5S, fraction 9) and the 40S and 60S X.l. ribosomal subunits as reference. Dots on the right margin correspond to reference proteins with 158, 116, 97, 66, and 56 kDa (from top to bottom). Note that most of the symplekin is recovered in particles with a peak value of ∼11S, whereas minor portions appear with a mean peak corresponding to ∼65S or in the pellet.