Fig. 5.

Differences in protein patterns in KO and WT cells. a The western blot for DDR-1 in the DDR-1 KO confirmed its absence; however, DDR-2 (b), MMP-13 (c) and collagen type I (d) were upregulated in the KO chondrocytes. Sox-9 (e), runx-2 (f) and COMP (g) were present in both the KO and WT cells. h β-actin staining confirmed the equal loading of the gels. i Coomassie blue staining was performed to evaluate the overall protein patterns. Protein isolation was performed using cells at passage 2 of the cells. Data are representatives of three individual experiments, or quantified as stated in “Results” (n = 6, including 3 KO mice and 3 WT mice, for western blots)