Fig. 1.
The isolation of inner from outer microdissected samples was evaluated by using specific markers to control for the absence of A tyrosine-hydroxylase (TH, 235 bp) transcript in the outer retina, and B of rhodopsin (179 bp) transcript in the inner retina by RT-PCR. Agarose gel electrophoresis of the transcript amplification products show that rhopdopsin transcripts are amplified from photoreceptor samples, whereas TH is not, and the inverse was found for TH transcripts in the inner retina for wild-type and Opn −/−4 mouse at all circadian times (CT) 0, 4, 8, 12, 16 and 20