Table 1.
Acrophase (±SD) of mRNA levels of clock and clock-controlled genes in the inner and outer retina, dissected by laser microdissection in the wild-type and Opn −/−4 mouse
| Acrophase (h) | ||||
|---|---|---|---|---|
| WT | Opn −/−4 | |||
| Gene | Inner retina | Outer retina | Inner retina | Outer retina |
| Per1 | 12.23 ± 1.32 | 23.55 ± 0.70* | – | 19.92 ± 1.50 |
| Per2 | 10.13 ± 1.92 | 17.27 ± 1.23* | 22.49 ± 1.57 | – |
| Per3 | – | – | – | – |
| Clock | 9.55 ± 1.36 | 10.42 ± 1.53 NS | 20.56 ± 0.95 | 13.33 ± 2.00# |
| Bmal1 | – | 5.3 ± 2.15 | 17.63 ± 1.66 | – |
| Cry1 | – | 1.26 ± 1.13 | – | – |
| Cry2 | – | 20.12 ± 1.10 | 6.33 ± 2.08 | – |
| Reverbα | – | 6.13 ± 1.75 | – | – |
| Rorβ | 1.71 ± 1.97 | 14.50 ± 1.50* | – | – |
| Dbp | 6.62 ± 2.01 | 1.96 ± 1.23* | 11.91 ± 1.45 | – |
| E 4 Bp 4 | 17.53 ± 1.66 | 6.79 ± 1.58* | 2.95 ± 1.42 | – |
Acrophase value is determined using cosinor analysis and test of amplitude 0 to assess goodness of fit. Only rhythms with a significant value are shown. In the wild-type mouse, for all the genes that present significant circadian expression in both the inner and the outer retina, the acrophases are significantly different between the two regions, with the exception of Clock; * p ≤ 0.05
In the Opn −/−4 mouse, only Clock presents a significant circadian expression in both the inner and the outer retina with an acrophase significantly different between both regions; # p ≤ 0.05