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LPL Nb5 prevents LFA-1 enrichment at the IS. a EGFP, LPL Nb5 or LPL Nb9 EGFP-tagged transfected Jurkat T cells were incubated for 45 min with SEE-pulsed Raji cells and subsequently stained for LFA-1 (alexa 594, red). The merged pictures show the T cell in green and the Raji cell in magenta (far red). Bar 10 μm. b Accumulation of LFA-1 in the IS was calculated from 20 cell couples per condition. The percentage of conjugated cells showing LFA-1 accumulation in the IS is depicted
