Fig. 6.

Expression of cell cycle regulators were decreased in hFis1-depleted cells. a Cells expressing each short hairpin RNA (shRNA) constructs were harvested at each time point after double thymidine block (DTB) release. Expressions of cell cycle regulators were analyzed by immunoblotting. The graph represents quantification of protein expression levels at 10 h after DTB release by normalization to actin (right). b Total RNA was isolated from each shRNA-expressing cell. Each messenger RNA (mRNA) level was detected by reverse-transcription polymerase chain reaction (PCR). GAPDH was used as an internal control. The graph represents quantification of cyclin B1, polo-like kinase1 (Plk1), or FoxM1 expression levels at 7 h after DTB release by normalization to GAPDH (right). * p < 0.05, ** p < 0.01 vs. control by Student’s t test