Fig. 5.

SnRNPs accumulate at CBs after reduction of immobile SMN by FGF-2²³. HEK293T cells were transfected with pFGF-2²³-ECFP (c1, c1’) or as controls pFGF-2¹⁸-ECFP (b1, b1’) or pECFP (a1, a1’) 24 h before fixation. Cells were fixed and immunostained with anti-coilin (a2–c2’) and anti-SmE (a3–c3’). Coilin and SmE showed colocalization in the nucleus as demonstrated in the merged images (a4–c4’) primarily in CBs. Arrows point to CBs that were enlarged. Intensity correlation analysis demonstrated in PDM (product of differences from the mean) images (a4’, b4’, c4’; LUT: orange, positive PDM; blue, negative PDM) showed a significant increase in colocalization of coilin and SmE in CBs after overexpression of pFGF-2²³-ECFP (M means ± SEM; *p < 0.05, ***p < 0.001; Mann-Whitney test; CFP: n = 45, FGF-2¹⁸: n = 45, FGF-2²³: n = 45, pooled from 3 independent experiments). Scale bar 10 μm (a, b, c), 2 μm (a’, b’, c’), 0.2 μm (PDM images)