Fig. 5.

LRP6 and WRCH1 are specific targets of miR-126. a Prediction of miR-126 target on LRP6 and WRCH1. Bioinformatic prediction was conducted using TargetScan. The base pairing between miR-126 and its potential target sites are listed. The “G–U” paring is labeled with “•”. The perfect matching between the seed region and seed-match region is boxed. b MiR-126 suppressed the expression of LRP6 and WRCH1. HeLa cells were transfected with miR-126 (0.1 nM) and infected with CVB3 as indicated. Cellular proteins were collected for WB analysis of the expression of LRP6 and WRCH1. The intensities of the bands were measured by ImageJ and the signal ratios are listed. c Validation of miR-126 targeting on LRP6 and WRCH1 by luciferase assay. HeLa cells were co-transfected with miR-126 or miR-CL and luciferase reporter vectors harboring the wt or mut targeting sites. Firefly and Renilla luciferase activities were detected by dual-luciferase assay and the firefly/Renilla luciferase ratios were calculated. All data were normalized to that of cells co-transfected with miR-CL and wt reporter vector (set as 1.0). p < 0.01, n = 4