Fig. 4.

P. aeruginosa triggers DNA strand breaks in infected cells. HL60 macrophages were infected at a MOI of 10 or 100 and analyzed using single-cell comet assay. Some mock slides were treated with hydrogen peroxide as a control of DNA breaks. a Typical representation of cells and comets with CometScore software. Comet tails are in red. b, c Quantification of comets. The percentage of DNA present in the comet tail is indicated and expressed as the mean ± SD of at least 60 comets (b). The percentage of cells with a comet tail is shown in c