Fig. 1.

DPP9-long form is expressed in HeLa cells. a Scheme of exon–intron structure of DPP9 showing the ATG for the long and short versions. b Protein sequence alignment of the short and long versions of DPP9. c Epitopes of antibodies used in this study. d Coomassie staining of various amounts of recombinant DPP9-l and DPP9-S, expressed and purified from Sf9 insect cells. e Defined concentrations of recombinant (rec.) DPP9-S and DPP9-l were separated on SDS PAGE and analyzed by Western blotting either with anti-DPP9 (long) antibody (upper gel) or with the DPP9 (total) antibody (lower gel). f Defined concentrations of recombinant (rec.) DPP9-l and total HeLa cell extracts were separated on SDS PAGE and analyzed by Western blotting with DPP9 (long) antibodies. g Total cell extract (10 μg per lane) from HeLa cells silenced for DPP9 using four different siRNAs were separated on SDS PAGE and analyzed with the DPP9 (long) antibody. Cells treated with non-targeting siRNA were used for control. Tubulin was blotted as a loading control. This assay was performed at least three times, shown is a representative blot