Fig. 4.

The N-terminal elongation of DPP9-L contains a functional nuclear localization sequence (NLS). a Protein sequence alignment of the N-termini of DPP9-S and DPP9-l with the NLS RKVKKLRL boxed in blue. b The NLS including the three for nuclear localization essential basic amino acids K5K6R8 targets DPP9-l to the nucleus. HA-tagged DPP9-l and various DPP9-l NLS mutants were overexpressed in HeLa cells and subjected to indirect immunofluorescence, detecting the HA tag and simultaneously RanBP2, a marker protein for the nuclear rim. Nuclei were visualized by Hoechst. Shown is a representative image from at least three independent experiments