Table 1.

Subcellular localization of DPP9-S, DPP9-l, and various DPP9 mutants

	Nucleus > cytosol (%)	Nucleus = cytosol (%)	Cytosol > nucleus (%)
DPP9-l	94.6 ± 7.9	5.4 ± 8.0	0 ± 0.1
DPP9-l ΔNLS	0 ± 0	0 ± 0	100 ± 0
DPP9-l K5AK6AR8A	0.2 ± 0.3	0 ± 0	99.8 ± 0.3
DPP9-l R2AK3A	94.9 ± 7.6	3.9 ± 6.2	1.2 ± 1.4
DPP9-l R8A	4.4 ± 6.1	36.8 ± 30.6	58.8 ± 32.7
DPP9-l R2A	96.3 ± 4.6	1.6 ± 1.5	2.0 ± 5.2
DPP9-S	0 ± 0	0 ± 0	100 ± 0
DPP9-S + NLS	92.5 ± 18.6	5.8 ± 13.9	1.7 ± 4.7

For quantification of subcellular localization, transfected cells were grouped into the following three categories: N>C (stronger signals in the nucleus), N=C (equal signal intensities in the nucleus and in the cytoplasm) and C>N (stronger signals in the cytosol). Quantification was performed from at least two independent experiments, from at least four slides with more than 340 cells expressing the respective DPP9 protein being counted