Fig. 3.

qPCR data for the expression of the D. melanogaster A-type mAChR gene CG4356 (A), and B-type mAChR gene CG7918 (B), in different body parts of adult male and female flies. The combined expression (long and short splicing variants) of the A-type and the combined expression (long and short splicing variants) of the B-type receptor were measured. At least 30 body parts were pooled for each mRNA isolation. These pools are the same for each body part column in A and B. The qPCR experiments were run in triplicate. The vertical bars in each column (which are sometimes smaller than the lines of the column) represent SEM. The mRNA concentrations given are relative to column a (a = 1). a female whole body; b female head; c female thorax; d female abdomen; e male whole body; f male head; g male thorax; h male abdomen. Note that both receptor genes are strongly expressed in the head (presumably the brain) of both males and females