Fig. 4.

qPCR data for the expression of the D. melanogaster A-type mAChR gene CG4356 (A) and B-type mAChR gene CG7918 (B) in different developmental stages. The combined expression (long and short splicing variants) of the A-type and the combined expression (long and short splicing variants) of the B-type receptor were measured. At least ten animals were pooled for each developmental stage. These pools are the same for each developmental stage in A and B. The sexes are mixed in columns a–e (they could not be discriminated), otherwise they are separated. The mRNA concentrations given are relative to column m (m = 1). Other conditions are as in Fig. 3. a 20–24 h old eggs; b larvae 2 days after egg laying(= first instar); c larvae 3 days after egg laying (= second instar); d larvae 4 days after egg laying (= second instar); e larvae 5 days after egg laying (= third instar); f male third-instar larvae; g female third-instar larvae; h 2-day-old male pupae; i 2-day-old female pupae; j 3-day-old male pupae; k 3-day-old female pupae; l adult male flies; m adult female flies. Note that both receptor genes are strongly expressed in older pupae of both sexes and in adult males