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. 2013 Jan 3;70(11):2015–2029. doi: 10.1007/s00018-012-1244-6

Fig. 1.

Fig. 1

FoxO3A accumulates into the mitochondria upon GR. a, b, c Upon GR (LG), FoxO3A accumulates into the mitochondria as shown by immunofluorescence analysis (a) in murine C2C12-derived (LG 24 h) and primary satellite cell-derived myotubes (LG 36 h), and by immunogold labeling (b) in murine C2C12-derived myotubes and NIH-3T3-fibroblasts (LG 24 h) (black dots represent gold particles recognizing FoxO3A immunocomplexes). These data (n = 3) were quantified by scoring the percentage of FoxO3A-positive cells and counting the number of gold particles per single mitochondria. Data are presented as mean ± SEM and significance was calculated with Student’s t test (c). d Cellular fractionation of murine NIH-3T3 and human primary IMR90 fibroblasts shows mitochondrial enrichment of FoxO3A under low glucose conditions (LG 16 and 24 h, respectively). The values indicated are the results of the densitometric analysis of FoxO3A normalized against the mitochondrial fractionation control VDAC (arbitrary units, HG = 1). Cellular fractionation controls: nuclei: PCNA; cytoplasm: GAPDH; mitochondria: VDAC. Asterisks non-specific band