Fig. 1.

Human KIF1Bβ isoforms. Schematic comparison of isoforms NM015074, AX039604 and AB088213 [the latter being representative of the AB088210–13 subgroup (AB)]. NM015074 (NM) and AX039604 (AX) share a characteristic C-terminus (red) to which an anti-peptide antibody was raised (indicated at the top). The AB series have an alternative C-terminus (denoted by the dark green box) not recognized by the antibody. AX and AB have an additional 6-aa stretch (black box) close to the motor domain, and AX also has a unique additional 40-aa stretch (gray box). The positions of the two sets of oligonucleotides that are each specific to NM or AX only, and can thus discriminate between NM and AX by PCR (Fig. 2b, d–f), are indicated by arrows over the areas to which they hybridize. The sequences of these oligonucleotide pairs are given in Online Resource Table S1. The motor (yellow), FHA (light blue), and PH domains (light green), as well as the two stretches of amino acids (black and gray boxes), unique in certain isoforms, are highlighted. Numbers denote aa positions