Fig. 6.

Quantitative MS analysis of glycosphingolipids after Gb3 synthase knockdown. HEp-2 cells were treated with non-targeting or Gb3 synthase siRNA, harvested and analyzed by mass spectrometry. The figure shows quantification of the major species of GlcCer (a), LacCer (b), and Gb3 (c) from one of two experiments. d The relative change in the total lipids of each class of glycosphingolipids after 24-h siRNA-mediated knockdown of Gb3 synthase. Previously obtained data of HG [34] are included for comparison