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Primers and probes used for qPCR 480 light cycler
| Gene | Primers (5′-3′) | Probe no. |
|---|---|---|
| BiP | FW: GCTGGCCTAAATGTTATGAGGA | 7 |
| RV: CCACCCAGGTCAAAC | ||
| CHOP | FW: AAGGCACTGAGCGTATCATGT | 21 |
| RV: TGAAGATACACTTCCTTCTTGAACA | ||
| hEEF | FW: CAATGGCAAAATCTCACTGC | 63 |
| RV: AACCTCATCTCTATTAAAAACACCAAA | ||
| Xbp-1 (unspliced) | FW: GACAGAGAGCCAAGCTAATGTGG | Sybr.Green |
| RV: ATCCAGTAGGCAGGAAGAT | ||
| Xbp-1 (spliced) | FW: AAGACAGCGCTTGGGGATGG | Sybr.Green |
| RV: CTGACCTGCTGCGGAC |
Probe numbers refer to numbers in the Roche universal probe library
