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. 2014 Feb 27;71(18):3599–3609. doi: 10.1007/s00018-014-1589-0

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Fig. 4 — Occludin is absent from the epidermis of K5Cre;ROSA26-Zeb2 ^tg/+neonates, leading to loss of function of the inside-outside epidermal barrier. a Intradermal injection of biotin and subsequent tracing with fluorescently labeled streptavidin shows that biotin diffusion in wild-type epidermis stops at the tight junctions of the granular layer, whereas in the K5Cre;ROSA26-Zeb2 ^tg/+ epidermis biotin passes through and reaches the outer surface of the epidermis. The “free ends” (arrows) marking the tight junctions formed between the stratum granulosum and the cornified envelope are absent in the Zeb2 transgenic epidermis. b Occludin is missing in the epidermis of P4.5 K5Cre;ROSA26-Zeb2 ^tg/+ pups. Claudin-1 is disturbed in the lower epidermal layers (white arrow) but not in the stratum granulosum, while ZO-1 is only mildly disturbed. Bars 20 μm (a) and 10 μm (b)