Fig. 6.

Analysis of the hOccludin promoter in MCF7/AZ cells. a Graphic overview of the human occludin promoter. The promoter fragment is approximately 440 bp and contains one of the two transcription initiation sites. The mutant construct carries the mutation of the E-box from CAGGTG to CAGTTG. b The ZEB2 expression plasmid was co-transfected in MCF7/AZ cells together with wild-type or mutant occludin promoter constructs. The graph depicts luciferase activity measured 48 h after transfection and the luciferase values are normalized against β-galactosidase expressed from a co-transfected plasmid. The values are scaled relative to the wild-type promoter activity. c The E-cadherin promoter was used as an experimental control. The bars represent the average of two independent transfection experiments in both b and c