Figure 3.

The effect on repression of the IME1 chromosomal region by lexA-Rme1p derivatives carrying different alterations in the zinc fingers (A) and alterations in the regions of RME1 that are proximal to zinc-finger regions (B). The Rme1 site repression was tested by the ability of different rme1 derivatives to repress sporulation in gal80Δ diploid strains carrying the wild-type RC. The lexA site repression was tested by the ability of different rme1 derivatives to repress sporulation via the lexA site placed at position −1950 bp in RC. Sporulation was scored after 48 h in liquid sporulation medium. IME2-LacZ expression was calculated after 24 h in sporulation medium.