Figure 5.

Effect of a series of N-terminal deletions on the ability to repress the endogenous IME1 gene and to activate a reporter plasmid. The Rme1 site repression was tested by the ability of gal80Δ diploids carrying different Rme1p derivatives to repress sporulation in strains with wild-type RC. Sporulation was scored after 48 h in liquid sporulation medium. IME2-LacZ expression was determined after 24 h in sporulation medium. Activation ability was determined by liquid β-galactosidase assays using pAC110–6 as a reporter.