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Transcriptional activation by Gal4-Rme1p fusion derivatives. The GAL4 DNA-binding domain was expressed as a fusion protein to the indicated Rme1p derivatives. The ability to activate transcription of ADE2 and HIS3 reporter genes in strain PJ69–4A was tested on SC medium (Rose et al., 1990) lacking adenine and on SC medium lacking histidine with or without addition of 3-AT.
