Table 1.
Yeast strains used in this study
| Strains | Genotype |
|---|---|
| AMP108 | α GAL80 RME1 |
| AMP714 | a his3 met4 |
| AMP764 | α his4-G IME2-lacZ-URA3 |
| AMP1122 | α rme1::PGAL1-S53-RME1::TRP1 |
| AMP1396 | α rme1::PGAL1-S53-RME1::TRP1 sin4Δ::TRP1 arg6 |
| AMP1420 | α rme1::PGAL1-S53-RME1::TRP1 his3ΔSK rgr1-100 |
| AMP1615 | a his3 |
| WL447 | a trp1::PGAL1-lexA-RME1::TRP1 |
| WL448 | a trp1::PGAL1-lexA::TRP1 |
| WL450 | a trp1::PGAL1-lexA-rme1-213::TRP1 |
| WL457 | a trp1::PGAL1-lexA-rme1-Cla179::TRP1 |
| WL458 | a trp1::PGAL1-lexA-rme1-Cla210::TRP1 |
| WL459 | a trp1::PGAL1-lexA-rme1-Cla239::TRP1 |
| WL460 | a trp1::PGAL1-lexA-rme1-Cla239,269::TRP1 |
| WL471 | a trp1::PGAL1-lexA-rme1Δ4-179::TRP1 |
| WL472 | a trp1::PGAL1-lexA-rme1Δ148-300::TRP1 |
| WL473 | a trp1::PGAL1-lexA-rme1Δ179-300::TRP1 |
| WL474 | a trp1::PGAL1-lexA-rme1Δ120-179::TRP1 |
| WL475 | a trp1::PGAL1-lexA-rme1Δ148-179::TRP1 |
| WL489 | a trp1::PGAL1-lexA-rme1Δ4-31::TRP1 |
| WL491 | a trp1::PGAL1-lexA-rme1Δ31-90::TRP1 |
| WL492 | a trp1::PGAL1-lexA-rme1Δ31-120::TRP1 |
| WL494 | a trp1::PGAL1-lexA-rme1Δ61-148::TRP1 |
| WL495 | a trp1::PGAL1-lexA-rme1Δ210-300::TRP1 |
| BPA71 | a/α trp1::PGAL1-lexA-rme1-213::TRP1/trp1 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 met4/MET4 |
| BPA80 | a/α trp1::PGAL1-lexA-RME1::TRP1/trp1 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 met4/MET4 |
| BPA86 | a/α trp1::PGAL1-lexA::TRP1/trp1 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 met4/MET4 |
| BPA101 | a/α trp1::PGAL1-lexA-rme1-Δ179-300::TRP1/trp1 met4/MET4 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 |
| BPA103 | a/α trp1::PGAL1-lexA-rme1-Δ148-179::TRP1/trp1 met4/MET4 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 |
| BPA105 | a/α trp1::PGAL1-lexA-rme1-Δ120-179::TRP1/trp1 met4/MET4 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 |
| BPA107 | a/α trp1::PGAL1-lexA-rme1-Δ148-300::TRP1/trp1 met4/MET4 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 |
| BPA110 | a/α trp1::PGAL1-lexA-rme1-Δ31-90::TRP1/trp1 met4/MET4 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 |
| BPA112 | a/α trp1::PGAL1-lexA-rme1-Δ31-120::TRP1/trp1 met4/MET4 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 |
| BPA113 | a/α trp1::PGAL1-lexA-rme1-Δ210-300::TRP1/trp1 met4/MET4 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 |
| BPA131 | a his3 met4 trp1::PGAL1-rme1Δ1-31::TRP1 |
| BPA132 | a/α trp1::PGAL1-rme1Δ1-31::TRP1/trp1 IME2-lacZ-URA3/IME2 his3/HIS3 his4-G/HIS4 met4/MET4 |
| BPA134 | a his3 met4 trp1::PGAL1-rme1Δ1-61::TRP1 |
| BPA135 | a/α trp1::PGAL1-rme1Δ1-61::TRP1/trp1 IME2-lacZ-URA3/IME2 his3/HIS3 his4-G/HIS4 met4/MET4 |
| BPA137 | a his3 met4 trp1::PGAL1-rme1Δ1-90::TRP1 |
| BPA138 | a/α trp1::PGAL1-rme1Δ1-90::TRP1/trp1 IME2-lacZ-URA3/IME2 his3/HIS3 his4-G/HIS4 met4/MET4 |
| BPA140 | a his3 met4 trp1::PGAL1-rme1Δ1-120::TRP1 |
| BPA141 | a/α trp1::PGAL1-rme1Δ1-120::TRP1/trp1 IME2-lacZ-URA3/IME2 his3/HIS3 his4-G/HIS4 met4/MET4 |
| BPA143 | a his3 met4 trp1::PGAL1-rme1Δ1-148::TRP1 |
| BPA144 | a/α trp1::PGAL1-rme1Δ1-148::TRP1/trp1 IME2-lacZ-URA3/IME2 his3/HIS3 his4-G/HIS4 met4/MET4 |
| BPA238 | a/α trp1::PGAL1-lexA-rme1-Cla179::TRP1/trp1 met4/MET4 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 |
| BPA240 | a/α trp1::PGAL1-lexA-rme1-Cla239,269::TRP1/trp1 met4/MET4 lexO-IME1/ime1Δ12-TRP1 IME2-lacZ-URA3/IME2 his3/HIS3 |
| PJ69-4A | a trp1-901 leu2-3,112 ura3-52 his3-2000 gal4Δ gal80Δ LYS::GAL1-HIS3 GAL2-ADE2 GAL7-lacZ RME1 (James et al., 1996) |
All the strains are SK-1 derivatives and carry the mutations leu2::hisG, trp1::hisG, lys2 ura3, ho::LYS2, gal80::LEU2, and rme1Δ5::LEU2, except where indicated otherwise.