Fig. 8.

The NES, NTS, and S778 are conserved between species and absent in Pyk2 isoform B. a Alignment of Pyk2 protein sequences from Homo sapiens, Anolis carolinensis, Meleagris gallopavo, Xenopus silurana, Danio rerio, Gasterosteus aculeatus together with the Homo sapiens isoform B lacking one exon. The NES, NTS, and S778 are shown with asterisk. The residues in the PKA consensus site are indicated in blue (−3, −2 basic) and green (+1 hydrophobic). b PC12 cells transfected with GFP-Pyk2 isoform A (long) or B (short) were treated with high K⁺ (K⁺, 40 mM) or control solution (C) for 5 min and analyzed by immunoblotting with anti-pY402 antibody (upper panel) or anti-Pyk2 antibody (lower panel). c PC12 cells transfected with GFP-Pyk2 isoform A or B (scheme on the left) were treated with high K⁺ (High K⁺) or control solution for 3 min. The percentage of cells with n ≥ c GFP fluorescence (middle) was quantified (right panel). Values are means + SEM, two-way ANOVA: isoform effect F _(1,12) = 261.83, p < 0.0001, K⁺ effect F _(1,12) = 118.57, p < 0.0005 interaction F _(1,12) = 6.42, p < 0.05. Newman–Keuls test: ***p < 0.001 versus control, °°°p < 0.001 versus WT. Scale bar 5 μm