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Schematic of yeast assay for the engineered StCas9 system. a Illustration of the yeast assay system. b Comparison of the scRNA and sgRNA.WT designs of SP1 protospacer and human CCR5 gene for guiding the StCas9 activity in yeast. Yeast transformation assay was performed with expression vectors for bStCas9, scRNA/sgRNA.WT bearing SP1 or CCR5 guiding sequence and corresponding surrogate reporter plasmids
