Fig. 2.

Apaf1 increases during early differentiation of primary cortical neurons (PCN) in vitro. a Primary cortical neurons (PCN) from WT mouse embryos at E13.5 were cultured for 2, 4, 8, and 12 day in vitro (DIV) and assayed for Apaf1, Tubb3, PSD95, Synaptophysin, Tau, and Gap43 mRNAs by quantitative real-time PCR. mRNA levels were normalized to L34 mRNA used as internal control. Data display the fold-changes of Apaf1, Tubb3, PSD95, Synaptophysin, Tau, and Gap43 mRNAs relative to 2 DIV PCN and are shown as the mean ± SEM.; n = 3, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.005 with respect to 2 DIV. b PCN from WT mouse embryos at E13.5 were cultured for 2, 4, 8, and 12 DIV and assayed for Apaf1 and PSD95 protein levels, using Gapdh as loading control. Density of immunoreactive bands was calculated using the software Image Lab (Bio-Rad), normalized for Gapdh, and reported as arbitrary units (shown as the mean ± SEM). *P ≤ 0.05, **P ≤ 0.01 with respect to 2 DIV