Fig. 4.

FBLN2 expression can be hormonally induced in mouse mammary gland. a Fbln2 mRNA levels relative to Krt18 in mammary glands of 4.5 weeks old mice that were hormone primed with either placebo, oestrogen-progesterone (E2 + P) or oestrogen (E2) pellets. Error bars show 95 % confidence levels. ***p value ≤0.001. n = 3 (placebo and E2 + P), n = 4 (E2). b Sections of mammary gland from 4.5 week old mice primed with placebo, E2+P and E2 hormone pellets for 1 week prior to tissue collection and stained with anti-FBLN2 antibody. No primary antibody control (−ve ctrl) shows no staining in the epithelium of E2 + P treated mice. Solid arrows denote TEB and dotted arrows indicate ducts. At least three mice were studied for each treatment. Scale bars are 100 μm