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a The zinc-finger molecule consists of one α-helix and two β sheets. The zinc ion (black ball) is bound by two cysteines of the β sheets and two histidines of the α-helix leading to a stabilization of the fold. Residues of the α-helix contact 3 bp of the DNA. b Four ZF molecules fused to a nuclease form a ZFN. Two ZFNs have to bind the targeted region in tail to tail direction to allow dimerization and cleavage of the FokI nuclease domain
