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a Immunoblots of brain (lane 1), heart (lane 2), lung (lane 3), and stomach (lane 4) rat homogenates showing expression of Nesfatin-1 and β-actin as housekeeping protein, b real-time PCR of Nucleobindin 2 mRNA expression in heart, lung, and brain, c Nesfatin-1 ELISA assay of heart, plasma and lung. Changes were evaluated as mean ± SE of six experiments for each group. Significant difference from control values (one-way ANOVA), *p < 0.05
