Fig. 5.

Lipid composition is changing during HEp-2 cell growth in the culture. Samples for lipid analysis by MS/MS were collected 1, 2, or 3 days after HEp-2 cell seeding to culture plate dishes. a The content of different lipid classes in the samples. Data are normalized to total lipid content detected for each time point. The error bars show the difference between the mean value and two independent biological replicas. b Relative change in lipid classes after 2 and 3 days in the culture compared to cells grown for 1 day. Abbreviations of all lipid classes are given under the section “Annotations of lipid species” in “Materials and methods”