Fig. 7.

Free cholesterol and cholesterol ester (CE) content in low and high density cells. a The content of free cholesterol was measured by Amplex^® Red Cholesterol Assay Kit in cell lysates from low and high density HeLa and HEp-2 cell cultures. The graph shows mean value of two independent experiments; the error bars represent the difference between the mean value and values of the two experiments. b The content of CE quantified by MS/MS in HEp-2 cells grown for 1, 2, or 3 days in the culture. The error bars show the difference between the mean value and two independent biological replicas. c Species distribution of CE in HEp-2 cells after 1, 2, or 3 days in the culture. d Relative change in CE species after 2 and 3 days in the culture compared to cells grown for 1 day