Fig. 2.

Cyclin A2 knockdown in NMuMG cells modulates 3D invasion, along with RhoA and RhoC activities. a Invasion of sh Luc and sh CycA2 NMuMG cells in a 3D collagen matrix (n = 5, ****P < 0.0001). b–d Western blotting after pull-down of the activated forms of Rac1 (b), RhoA (c) or RhoC (d) in extracts of cells depleted of Cyclin A2. Lower panels Quantification where the amount of the GTP-bound form was normalized to total Rac1, RhoA, RhoC and GAPDH. Data are represented as mean ± SEM (*P = 0.01 for RhoA, n = 3 and *P = 0.01 for RhoC, n = 3). e Co-immunoprecipitation of endogenous Cyclin A2 with RhoC. Cell lysates obtained from NMuMG cells transfected with RhoC-GFP were incubated by GFP-Trap beads. Immunoprecipitates were subjected to SDS-PAGE and analyzed by Western blotting with an anti-Cyclin A2 antibody