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. 2014 May 31;71(24):4881–4894. doi: 10.1007/s00018-014-1654-8

Fig. 4.

Fig. 4

Impact of RhoA and RhoC knockdown ± that of Cyclin A2 in NMuMGRasV12 cells. a Invasion in collagen matrix (3D) of NMuMGRasV12 cells knocked down for both RhoC and Cyclin A2 (left panel) or for RhoA alone (right panel) (n = 3, ****P < 0.0001 for siRhoC and n = 3, ***P < 0.0002 for siRhoA). b Western blot analysis of E-Cadherin and RhoA depletion in NMuMGRasV12 cells. c, Analysis of E-Cadherin and p120 Catenin cellular localization in NMuMG and NMuMGRasV12 cells treated with siRNA RhoA. qPCR analysis of mRNA levels of EMT markers in NMuMGRasV12 cells knocked-down for RhoA (d) or both RhoC and Cyclin A2 (e). Results are shown as fold change of siRNA of interest knockdowns over control knockdowns (NMuMGRasV12 cells were used for the siRhoA and sh CycA2NMuMUGRasV12 cells for siRhoC). Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001