Fig. 6.

Cyclin A2 knockdown modulates extravasation and migration in avian embryos. a Extravasation of sh Luc and sh CycA2 NMuMG cells with or without RasV12 expression. The data represent the percentage of cells present in the stroma versus the total cell number detected in the ROI. b Velocity of sh Luc and sh CycA2 NMuMGRasV12 cells expressed in microns traveled per hour. c Displacement of sh Luc and sh CycA2 NMuMGRasV12 cells expressed in microns traveled away from the origin. d Migration tracks (red line) of sh Luc and sh CycA2 NMuMGRasV12 cells within CAM stroma as analyzed by Volocity software. e Image set of sh Luc and sh CycA2 NMuMGRasV12 cells present in the stroma. Images were acquired every 15 min for 12–16 h. Data are represented as mean ± SEM. *P < 0.05