Fig. 5.

Assessment of PLB1_Triple and PLB1_WT in the open-field water maze. Mean ± SEM. a Acquisition phase for 4- and 12-month-old mice showing the mean distance required to find the platform on each day of testing including training with a visible platform (V). Note the markedly slower rate of learning in PLB1_Triple mice at 12 months (right). Beneath each graph are representative sample swim paths from individuals over the course of the training days. b Time in target quadrant during the probe trial (60–90 min post-training) was not different between genotypes at any age. c Number of crossings of former platform location during probe test also did not show a phenotype. Note, older animals presented with lower number of platform crossings compared to young mice, but this was only reliable for the WT group (*p < 0.05). n.s. not significant