Fig. 5.

Effects of TDB on CK2 and Pim-1 endocellular activity. a HEK-293T cells were transfected with Akt in order to better detect the CK2 target site Ser129 of Akt [45]. Transfected cells were treated for 6 h with increasing concentrations of TDB. Cell lysate proteins (10 μg) were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control. b CEM cells were treated for 24 h with increasing concentrations of TDB. Cell lysate proteins (20 μg) were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control