Table 2.

Comparison of the accessibility of amino acids from TM6 and TM12, deduced from our simulation (SIM) and from experience (EXP)

Experimental results correspond to those obtained by cysteine scanning using channel-permeant and channel-impermeant probes, as described in [21]. Amino acids are predicted or observed to be (•) or not to be (−) accessible. SB stands for involved in salt bridge. In bold and italics are indicated positions with discrepancy. Positions within the limits of the crossing bridge are shaded gray. The references of conflicting results obtained by other, independent experimental studies are labeled 1: [68], 2: [66] and 3: [67]. Position indicates the position of the amino acids within the model of the 3D structure

b buried, Extra extracellular loop, ExV extracellular vestibule, UpP upper part of the pore, 5 secondary, “pentagonal” duct, 6 main “hexagonal” pore, InV inner vestibule