Fig. 1.
Quantification of the internalisation of the CPPs, studied in the presence and the absence of SMase, in WT and GAGneg cells at 37 °C. 106 cells were incubated with the peptides in the presence or the absence of 1 U SMase, in 1 mL DMEM, at 37 °C for 70 min. The amounts of internalised peptides (µM) were then quantified using a MALDI-TOF MS reported protocol [27, 28]. Experiments were done in duplicate or triplicate, and repeated at least three times independently. Significance was tested using a Welch’s corrected t test (ns p > 0.05, *0.05 < p > 0.01, **0.01 < p > 0.001, ***p < 0.001)