Table 2.
Impact of cell surface treatment on internalisation of CPPs in wild type and GAGneg cells
| Peptide | Wild-type | GAG-deficient | |||||
|---|---|---|---|---|---|---|---|
| SMase | HI, HIII, ChABC | SMase, HI, HIII, ChABC | MβCD | Control | SMase | MβCD | |
| R6L3 | 2 | – | – | – | 1 | 1 | – |
| Tat | 3 | – | – | 1 | 1 | 1 | 1 |
| R9 | 3 | – | – | – | 0.35 | 0.65 | – |
| penetratin | 6 | 0.75 | 0.71 | 2 | 0.60 | 0.40 | 1 |
| R6W3 | 8 | – | – | – | 0.70 | 1.35 | – |
Cells were incubated with 10 μM peptide. Data are normalised for every peptide to the quantity measured in control wild-type cells
SMase sphingomyelinase, HI heparinase I, HIII heparinase III, ChABC chondroitinase ABC