Fig. 3.

Inhibition of the wound closure response by syndecan-1 knockdown in A549 cells. A549 cells were transduced with lentivirus encoding scramble (scr) or SDC-1 shRNA. a–b Efficiency of downregulation was controlled by flow cytometry (a) and quantitative PCR (b). c Transduced cells were assayed for changes in confluence over 48 h of culture using the IncuCyte ZOOM software. d–e Transduced A549 cells were grown to confluency on coated collagen G (d) or fibronectin (e) and wounded by a defined scratch. Wound closure was monitored from 0 h after scratch induction to 24 h and quantified using the IncuCyte ZOOM software. f–g Transduced cells were wounded by scratching and subsequently covered with matrigel to analyze cell invasion from the wound edges into the matrigel within the wounded area (f). Exemplary images of three independent invasion experiments are shown (g). Quantitative data were calculated as means + SD from three independent experiments. Statistically significant differences compared to scramble control are indicated by asterisks (p < 0.05)