Fig. 6.

Restoration of intracellular signaling by overexpression of syndecan-1 tCTF in A549 cells lacking endogenous syndecan-1. A549 cells were transduced with scr or SDC-1 shRNA vector. Subsequently, a second transduction was carried out with virus for overexpression of SDC-1 tCTF or with empty vector control virus. Cell lysates of double transduced A549 cells were analyzed for phosphorylation or activation status of ERK (a), p38 (b), Akt (c), Rho GTPase (d), and FAK at sites Tyr397 (e) and Tyr925 (f) by Western blotting. Signals were quantified by densitometry as phosphorylated or activated versus total forms and calculated in relation to the appropriate control cells. Data represent means + SD of three independent experiments. Asterisks indicate significant differences to the scramble control, hashes indicate significances due to overexpression of the tCTF (p < 0.05)