Fig. 6.

Differential gene expression in biopsied and control 4.5-dpc embryos (n = 3 each). a Microarray analysis. Differentially expressed genes mainly participate in DNA methylation, chromatin remodeling, cell cycle, oxidative stress, embryo development, and cell differentiation. b DAVID analysis indicated that the transcripts upregulated in the biopsied embryos mainly related to “metabolic process” and the “response” to various stimuli (upper), and the downregulated transcripts in the biopsied embryos mainly associated with cell cycle events and transcriptional regulation (nether). c Real-time PCR confirmation (*p < 0.05)