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. 2002 May;13(5):1750–1764. doi: 10.1091/mbc.01-12-0592

Figure 9.

Figure 9

CT transport to the ER is inhibited by Arf1(Q71L) and Sar1(H79G). Vero cells were injected with a mixture of GFP and either Arf1(Q71L) (A and B) or Sar1(H79G) (C–F) expression plasmids and incubated in 10 μg/ml cycloheximide for 4 h. The cells were then allowed to bind and internalize CT holotoxin in the absence of cycloheximide at 20°C for 1 h and subsequently at 37°C for a further 2 h. Although the toxin (B, D, and F) clearly reached the ER in uninjected cells (outlined in A, C, and E), cells expressing the Arf1 or Sar1 mutants (identified by GFP expression shown in A, C, and E) displayed a conspicuous lack of toxin in the ER. Instead, an accumulation of the toxin in endosomes and sometimes the Golgi was observed in injected cells. Bars, 10 μm.