Fig. 2.

NADPH oxidase/NOX2 activation in the cell-free system. The phagocyte NADPH oxidase can be activated in a reaction mix containing cytosol, which provides cytosolic components (p47phox, p67phox and Rac1/2), plasma membranes (which provide cytochrome b558) isolated from resting cells (neutrophils, monocytes or macrophages), Mg⁺⁺, GTP and an anionic amphiphile, such as arachidonic acid or sodium/lithium dodecyl sulfate (SDS, LDS). Although p40phox is present in the cytosol and in the complex, this protein is not necessary for NADPH oxidase activation in the cell-free system (FRO=ROS)