Fig. 3.

Chemotactic movement of coronin mutants is altered. a Computer generated cell tracks and stack images of all coronin mutants in comparison to the wild-type Ax2. Aggregation competent cells were deposited on coverslips and after settling down challenged with cAMP. The star indicates the location of the pipette filled with cAMP. The cell perimeters were outlined and are shown for two representative cells each. The data for Ax2 and corB ⁻ are from Shina et al. [16]. Bar 8 μm. b Cell shape changes of aggregation competent mutant and Ax2 cells were analyzed by DIAS software. Images were taken every 10 s. The outlines were traced manually and the changes of direction (arrows) calculated using the DIAS image analysis software. The green areas indicate new membrane protrusions and the red areas retractions