Fig. 3.

Detection of key basic residues in NLS2 for nuclear import. a Sequence of the putative NLS2 is shown. Two segments of either residues 169–193 or residues 169–204 were fused to EGFP. The basic amino acids lysine and arginine (in boldface) in residues 169–204 were substituted by alanines as indicated by arrows. The subcellular localization of LRH-1 mutants is summarized on the right (N nuclear, C cytoplasmic). b Wild-type and mutant forms were transfected into COS-7 cells. After 24 h, cells were fixed and the nuclei counterstained with DAPI. Fusion proteins were visualized by confocal microscopy. Scale bar 25 μm