Fig. 7.

Comparison with other nuclear receptors and the structure of LRH-1 NLS domain. a Sequence alignment of the zinc-finger domain and the adjacent region of several nuclear receptors. The cysteine residues that coordinate zinc binding are highlighted by grey boxes. The nuclear localization domains NLS1 and NLS2 of LRH-1 are indicated. The conserved basic amino acids that were mutated in this study are highlighted by dark boxes. The key basic amino acids identified for nuclear import of NLS1 and NLS2 are indicated by asterisks. b Diagram of the zinc binding loops of LRH-1. The unique Ftz-F1 box for NR5A subfamily members is indicated